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CL-387785 affects CD80 <t>transcription</t> by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.
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CL-387785 affects CD80 <t>transcription</t> by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.
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CL-387785 affects CD80 <t>transcription</t> by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.
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CL-387785 affects CD80 <t>transcription</t> by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.
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CL-387785 affects CD80 <t>transcription</t> by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.
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CL-387785 affects CD80 <t>transcription</t> by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.
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CL-387785 affects CD80 transcription by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.

Journal: International Journal of Biological Sciences

Article Title: Targeted Induction of Cancer Cell Necroptosis Potentiates Anti-PD-1 Immunotherapy via CD80 Activation

doi: 10.7150/ijbs.121690

Figure Lengend Snippet: CL-387785 affects CD80 transcription by regulating the nuclear translocation of NF-κB. (A) Western blotting was used to detect the protein expression of P65 and P50 in SK-MEL-5, A375 and A549 cells. (B) Chip-PCR (n=3) and luciferase reporter gene assays (n=4) were used to examine the mechanism underlying the transcriptional regulation of CD80 by P65. (C) The clinical significance of CD80 expression in the melanoma and lung cancer cohorts.

Article Snippet: Total RNA (3 μg) served as a template for the reverse transcription reaction (RT Master Mix for qPCR II; HY-K0511A; MedChemExpress, USA).

Techniques: Translocation Assay, Western Blot, Expressing, Luciferase